This experiment was attempted in order to determine the optimum concentration of plant growth regulators for callus formation and plantlet differentiation from tissue of female sexual organ, stigma, style and ovary, of Ornithogalum thyrsoides in vitro.
The results obtained were summarized as follows.
1. Stigma and ovary tissue of female sexual organ was identified as good materials for proliferation of plantlets.
2. In stigma culture, callus was farmed in the medium with NAA, IAA or kinetin combined with 2, 4-D, but plantlet was not differentiated, in these media.
3. Callus originated from stigma tissue was milky white like cream, while those induced from style and ovary being white yellow were friable or solid.
4. The medium supplemented with 0.2㎎/ℓ NAA and 2.0㎎/ℓ kinetin was identified as best for both callus proliferation and plantlet differentiation of different tissues tested.
|